Abstract: Previous studies of mafic microgranular enclaves (MMEs) and host granite have revealed that crust-mantle interaction plays an important role in granitic magma formation. However, whether MMEs and host granite are the products of different evolutionary stages of homologous magmas needs further exploration. In this study, the MMEs from the Ziyunshan pluton were separated and their zircon U-Pb Hf-O isotopic compositions were analyzed to reveal their petrogenesis and the genesis relationship with the host granite. U-Pb age of the MMEs is 225.1±5.3 Ma, which is in general agreement with the age of their host granite (225.2±1.7 to 227.0±2.2) Ma of their host granites. The ε_Hf(t) and δ¹⁸O values of the MMEs are -6.4 to -1.6 and 7.6‰ to 9.9‰, respectively, that are consistent with Hf-O isotope composition of their host granite. Two-stage model ages (T_DM2) of the MMEs range from 1.35 to 1.65 Ga (average 1.51 Ga), which is slightly higher than that of the host granite (1.22 to 1.79Ga, average 1.36 Ga). The results indicate that the Ziyunshan MMEs and host granite are formed in post-collision tectonic setting. The initial magma chamber was firstly formed by the mantle magma and the anatectic magma in the lower crust, and the upper highly evolved felsic magma and a small amount of lower low-evolved ferrimafic magma emplaced to shallow strata. This study would provide a new perspective for understanding the genetic relationship between MMEs and theri host granites.